RNA

Part:BBa_K115017:Experience

Designed by: Bastiaan van den Berg   Group: iGEM08_TUDelft   (2008-08-20)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K115017

In our experiments, using BBa_K115035 (a Renilla luciferase expressing device) for testing of this RNA thermometer, we showed K115017 causes a 4-fold repression of translation in comparison to the RBS BBa_B0032.

The parts were tested in luciferase constructs, by measuring luciferase activity after growing at certain temperatures. The fold increase of luciferase activity between cultures of cells grown at 20ºC and 37ºC is displayed in figure 1.

In this figure, 12 (the leftmost) is construct K115012, the reference strain, which has no thermosensitive RNA, but just B0032 as ribosome binding site. This is to compare for normal temperature induced behaviour in cells. The other constructs from 29-36 are K115029 to K115036, please look at the experience tab of these constructs to see which thermometer RNA they contain.

Figure 1. Fold increase of luminescence per ug of total protein of each construct in respect to luminescence measured at 20ºC. Numbers in the legend represent the last two numbers of the construct name, e.g. 12 = BBa_K115012. Four samples were measured in duplo for every data point. Error bars represent two times SEM.


For more documentation, visit our wiki [http://2008.igem.org/Team:TUDelft (link)].

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